Switching on RNA silencing suppressor activity by restoring argonaute binding to a viral protein.

نویسندگان

  • Edit Z Szabó
  • Máté Manczinger
  • Anikó Göblös
  • Lajos Kemény
  • Lóránt Lakatos
چکیده

We found that Sweet potato feathery mottle virus (SPFMV) P1, a close homologue of Sweet potato mild mottle virus P1, did not have any silencing suppressor activity. Remodeling the Argonaute (AGO) binding domain of SPFMV P1 by the introduction of two additional WG/GW motifs converted it to a silencing suppressor with AGO binding capacity. To our knowledge, this is the first instance of the transformation of a viral protein of unknown function to a functional silencing suppressor.

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عنوان ژورنال:
  • Journal of virology

دوره 86 15  شماره 

صفحات  -

تاریخ انتشار 2012